Carbohydrates downregulates sodium‐dependent neutral amino acid transporter‐2 expression (946.5)
نویسندگان
چکیده
منابع مشابه
Simultaneous analyses of neutral carbohydrates and amino sugars in freshwaters with HPLC-PAD.
In this study, we determine concentrations of neutral and amino sugars and a sugar alcohol in freshwaters using high-performance liquid chromatography and pulsed amperometric detection with a single isocratic analysis. Coeluting arabinose, galactosamine, and mannosamine are separated with a mobile phase of 22.8 mM NaOH-KOH at a temperature of 17 degrees C. The resolutions are 0.73 and 0.64, res...
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In this report, we demonstrate the expression of the mammalian System A neutral amino acid transporter in Xenopus laevis oocytes following microinjection of mRNA from rat liver, Chinese hamster ovary (CHO) cells, and human placenta. Stage 6 oocytes were injected with poly(A+) mRNA from one of these three sources and incubated for 24 h prior to assaying Na(+)-dependent 2-aminoisobutyric acid tra...
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Amino acid supply in brain is regulated by the activity of the large neutral amino acid transporter (LAT) at the brain capillary endothelial cell, which forms the blood-brain barrier (BBB) in vivo. Bovine BBB poly(A)(+) RNA was isolated from 2.0 kg of fresh bovine brain and size fractionated on a sucrose density gradient, and a size-fractionated bovine BBB cDNA library in the pSPORT vector was ...
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Absorption of neutral amino acids across the luminal membrane of intestinal enterocytes is mediated by the broad neutral amino acid transporter B0AT1 (SLC6A19). Its intestinal expression depends on co-expression of the membrane-anchored peptidase angiotensin converting enzyme 2 (ACE2) and is additionally enhanced by aminopeptidase N (CD13). We investigated in this study the expression of B0AT1 ...
متن کاملExpression cloning of a Na(+)-independent neutral amino acid transporter from rat kidney.
Uptake of long-chain and aromatic neutral amino acids into cells is known to be catalyzed by the Na(+)-independent system L transporter, which is ubiquitous in animal cells and tissues. We have used a Xenopus oocyte expression system to clone the cDNA of a system L transporter from a rat kidney cDNA library. The 2.3-kilobase cDNA codes for a protein of 683 amino acids. The transporter has four ...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2014
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.28.1_supplement.946.5